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KMID : 0613820150250040397
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Journal of Life Science
2015 Volume.25 No. 4 p.397 ~ p.405
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Inhibitory Effects of S-allylcysteine on Cell Proliferation of Human Cervical Cancer Cell Line, HeLa
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Kim Hyun-Hee
Min Gye-Sik
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Abstract
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S-allylcysteine (SAC) is a water-soluble organosulfur compound abundant in the aged garlic extract and has been drawing attention as a diet-derived alternative agent not only for the effects of anti-oxidation and anti-inflammation but also for the prevention and treatment of various types of cancer. However, there is no report about the anticancer effects of SAC on cervical cancer cells. The aim of this study was to analyze the inhibitory effects of SAC on cell proliferation of cervical cancer cell line, HeLa and to examine its effects on the apoptosis and cell cycle as the cellular mechanisms of anti-proliferation. For this, we examined effects of different concentrations of SAC on cell proliferation according to treatment periods. Treatment with SAC not only induced morphological changes but also resulted in the reduction of cell viability and the inhibition of concentration- and time-dependant cell proliferation of HeLa. Furthermore, SAC also induced fragmentation of DNA in both DNA fragmentation and TUNEL assays, and induced cell cycle arrest at the G2/M phase in cell cycle analysis. These results suggest that SAC inhibits proliferation of HeLa at least in part through the induction of apoptosis and the cell cycle arrest.
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KEYWORD
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Apoptosis, cell cycle, human cervical cancer cell (HeLa), proliferation, S-allylcysteine (SAC)
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